|
KMID : 0545119980080020141
|
|
Journal of Microbiology and Biotechnology
1998 Volume.8 No. 2 p.141 ~ p.145
|
|
|
Overproduction and Secretion of β-Glucosidase in Bacillus subtilis
|
|
Kim, Jeong Hyun
Lee, Baek Rak/Pack, Moo Young
|
|
|
Abstract
|
|
|
|
Overproduction of intracellular β-glucosidase was attempted by modifying the promoter region of a β-glucosidase gene cloned from Cellulomonas fimi and expressing it in Bacillus subtilis DB104. A strong engineered promoter, BJ27UΔ88, was fused to the β-glucosidase gene after removing its native promoter. An effective Shine-Dalgarno sequence (genel0 of phage T7) was inserted between the promoter and the P-glucosidase structural gene. The modified gene was overexpressed in B. subtilis and produced 1121.5 units of β-glucosidase per mg protein which is about 12% of total intracellular protein. Secretion of overproduced intracellular β-glucosidase was attempted by using the signal sequence of the Bacillus endoglucanase gene as well as an in-frame hybrid protein of endoglucanase. The hybrid protein was normally secreted into the culture medium and still retained β-glucosidase activity.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|